Yes. You can use transaction SU10 which allows you to select users based upon a number of criteria (usergroup, role, profile, etc.) and assign them or modify a number of user attributes. Additionally, If your company has also implemented SAP HR-OM, and you have taken advantage of Infotype 105 (Pernr to User-id relationship) you could assign a role at any point in the org structure (provided with minimal modification to evaluation path PROFL0), as a result any employee that was connected to that org unit would receive the role ( you must be using RHPROFL0 or RHAUTUPD_NEW).
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